High-Throughput of 28 Pesticide Residues in Strawberry and Lettuce (Copure® 24-Well Pesticide Residue Purification Plates)

Introduction

QuEChERS is widely used to detect pesticide residuesin plant-based foods. Biocomma introduces the Copure®24-Well Pesticide Residue Purification Plates enablingrapid, high-throughput analysis with Biocomma® PositivePressure 24 Processor system and sample vial tray. Nosyringe filtration is required prior to LC-MS/MS analysis.Biocomma® established a high-throughput LC-MS/MS method with simplified operation and betterresult stability for detecting 28 pesticide residues instrawberries and lettuce. The recoveries were 60-110%with CV values below 10% at both 5 ng/g and 10 ng/gconcentrations, which is close to traditional QuEChERSmethods for your reference.

Experiment

Extraction (Copure® QuEChERS, Cat. #: COQ050010H)

Chop the samples and grind evenly. Weigh 10.0 g ofsample into a 50 mL centrifuge tube. Add 10 mL ofacetonitrile and vortex mix for 5 min. Add a pack ofQuEChERS extraction salt and shake vigorously for 1min. Vortex for 5 min, then centrifuge at 5000 r/min for 5min. The upper acetonitrile layer is ready for purification.

Purification (Copure® 24-Well Pesticide ResiduePurification Plates, Cat. #: NC24002)

Place the 24-well purification plate on a Biocomma®24-Well Vial Tray. Add 2 mL of prepared sample. Placethe purification plate and vial tray on the Biocomma®Positive Pressure 24 Processor. Turn on the gas. Makesure the wells of purification plate are positioned directlyunder the gas vent. Adjust the gas flow until all thesample filter into the sample vials. Screw on the capsand the sample is ready for analysis.

Instrument Conditions

1.Chromatography ConditionsEquipment: UPLC-MS/MS (Thermo Scientific TSQEndura)Chromatographic column: Commasil® BEH T-C18 (100mm×2.1mm, 3μm)Mobile phase: A: Water (0.1% formic acid), B: MethanolFlow rate: 0.3 mL/minInjection volume: 5 µLColumn temperature: 30 ℃

Table 1. Gradient Elution Program

2.Mass Spectrometry Conditions

Source of ion: HESIElectrospray voltage: 3500VSheath gas pressure: 40 ArbAuxiliary air pressure: 1 ArbIon transfer tube: 380 ℃Atomization temperature: 350 ℃

Results

Table 2. Experimental Results of Spiked Recovery