biocommaIntroduction
Mycotoxins, produced by molds or fungi during food orfeed growth, are harmful secondary metabolites. Over 300mycotoxins exist naturally, with aflatoxin (AFT), zearalenone(ZEN), and T-2 toxin posing risks to livestock quality. Humaningestion leads to liver damage, cancer, teratogenicity, andimmune diseases. Biocomma established a rapid LC-MSmethod using Copure® 226 Multifunctional Purification Columnsfor detection of aflatoxin B1, ZEN, and T-2 toxins in feed withgood recovery and stability at three different concentrations foryour reference.
Experiment
Extraction
Weigh 5.0 g of the sample into a 50 mL centrifuge tube, andadd 25 mL of acetonitrile-water solution (84:16). Vortex for 3min. Then, extract by ultrasonic treatment for 20 min. Shake 2-3times during this period. Centrifuge at 10000 r/min for 5 min.The sample is ready for purification.
Purification (Copure® 226 Multifunctional PurificationColumn)
Add 10 mL of prepared sample to a glass tube. Insert therubber head of the purification column into the tube, and pushto the bottom. Pipette 5 mL of the purified sample to a samplevial or EP tube. Evaporate sample to dryness and redissolveby 1 mL of 20% acetonitrile solution. Vortex to dissolve theresidue. After filter by 0.22 μm microporous membrane, thesample is ready for analysis.
Preparation of Standard Curve Solution
Prepare blank sample in the same procedures. Add anappropriate amount of standard solution. Evaporate to drynessat 45 °C and dilute by 1 mL of 20% acetonitrile solution toprepare the concentration of 2 ng/mL, 5 ng/mL, 10 ng/mL, 20ng/mL, 50 ng/mL, and 100 ng/mL.
Instrument Conditions
1.Chromatography Conditions
Instrument: Thermo Fisher TSQ Endura
Chromatographic column: Elite SinoPaK BEH T-C18 (2.1mm×100 mm,3 μm)
Mobile phase: A: 5 mmol/L Ammonium acetate solution, B:Methanol (contains 0.1% formic acid)
Flow Rate: 0.3 mL/min
Column Temperature: 30℃
Injection volume: 5 μL
Table 1. Gradient Elution Program
2.Mass Spectrometry Conditions
Ion source: HESI
Scan mode: positive ion (ESI+) and negative ion mode (ESI-)
Sheath gas pressure: 30 arb
Auxiliary gas pressure: 8 arb
Ion exchange tube: 300 ℃
Auxiliary gas temperature: 350 ℃
Table 2. Targets and Characteristic Ions (*Quantitative Ions)
Results
Table 3. Spike Recovery
Figure 1. EIC of Targets at 10.0 μg/kg
Ordering Information
