biocommaIntroduction
Aflatoxin, a secondary metabolite synthesized by fungi such asAspergillus flavus and Aspergillus parasiticus via the polyketidepathway, typically comprises a difuran ring and an oxinone.Natural aflatoxins B1, B2, G1, and G2 are classified basedon their chemical structures. These mycotoxins, present incontaminated crops used for livestock feed, can accumulate inmeat, eggs, milk, and other foods, posing health risks throughthe food chain.
Biocomma established a rapid LC-MS/MS method usingCopure® 228 Multifunctional Purification Columns for detectionof aflatoxins B1, B2, G1, and G2 in corn flour with goodrecovery and stability for your reference. The recoverieswere 90-100% with RSD less than 5% at three differentconcentrations. This method is also applicable to cereals,cereal products, nuts, seeds, oils, condiments, infant formulas,and supplementary foods.
Experiment
Extraction
Weigh 5 g of sample into a 50 mL centrifuge tube. Add isotopeinternal standard and vortex to mix well. Let stand for 30 min.Add 20 mL of acetonitrile-water solution (84+16) and vortexto mix well. Then, extract by ultrasonic treatment for 20 min.Centrifuge at 6000 r/min for 10 min. The supernatant is readyfor purification.
Purification (Copure® 228 Multifunctional PurificationColumn)
Add 10 mL of supernatant to a glass tube. Insert the rubberhead of the purification column into the tube, and push to thebottom. Pipette 5 mL of the purified sample to a sample vial orEP tube. Evaporate sample to dryness and redissolve by 1 mLof initial mobile phase. Vortex for 30 seconds to dissolve theresidue. After filter by microporous membrane, the sample isready for analysis.
Instrument Conditions
1.Chromatography Conditions
Equipment: LC-MS SCIEX Triple Quad™ 4500Chromatographic column: Waters C18 (2.1 mm×100 mm,1.7um)
Mobile phase: A:0.1% Formic acid water, B: AcetonitrileMobile phase gradient: initial 80%A, 20%A (0 min~2 min),20%A (2 min~3 min), 80%A (3 min~3.1 min), 80%A (3.1 min~5min).
Flow rate: 0.3 mL/min
Column temperature: room temperature
Injection volume: 10.0 L
2.Mass Spectrometry Conditions
Detection mode: MRM
Table 1. Ion Source Control Conditions
Table 2. Ion Selection Parameters (*Quantitative Ions)
Results
Table 3. Spiked Aflatoxin in Corn Flour Recovery
Based on Figure 1, the pigments are obviously absorbed by Copure® 224 Multifunctional Purification Column. Thedecolorization ability is similar to Brand A and much better than Brand B.
Ordering Information
