biocommaIntroduction
Peanuts are susceptible to mold contamination, particularlythe presence of aflatoxins like Aflatoxin B1 (AFB1), whichsignificantly diminish nutritional and commercial value.Furthermore, AFB1 contamination poses a substantial threatto the edibility and safety of peanuts and peanut products. Theimmunoaffinity method offers distinct advantages, includingprecise results, high recovery rates, and excellent precision.Biocomma established an immunoaffinity UPLC-MS/MSmethod for detection of AFB1 in peanut by Copure® AflatoxinB1 Immunoaffinity Columns with good recovery and precisionfor your reference. The recoveries were 90-110% with RSDless than 5%.
Experiment
Extraction
Weigh 5.00 g of ground peanuts into a 50 mL centrifuge tube.Add 100 μL of internal standard, then 20 mL of acetonitrilewater solution (84+16) and vortex to mix well. Extract byultrasonic treatment for 20 min. Centrifuge at 8000 r/min for5 min. Pipette 4.0 mL a clean tube, and add 20 mL of PBSsolution. After mixed well, the sample is ready for purification.
Purification (Copure ® Aflatoxin B1 ImmunoaffinityColumns, 3 mL)
Set the immunoaffinity column on the biocomma® SPE VacuumManifold. Add the prepared solution at flow rate of 1~2 drop/s. Then add 5.0 mL PBS buffer and 5.0 mL water. Drain thecolumn. Add 1.0 mL of methanol. Close the valve and soak for30 s, then elute into a sample vial for LC-MS/MS analysis.
Preparation of Procedural Blank
Carry out the experiment according to the above steps withoutsample.
Instrumental Conditions
Chromatographic column: Commasil® T-C18 (2.1 mm×100 mm,3 μm)
Mobile phase A: 5 mmol/L ammonium acetate
Mobile phase B: Methanol (containing 0.1% formic acid)
Flow rate: 0.3 mL/min
Column temperature: 30 ℃
Injection volume: 5 μL
Table 1. Gradient Elution Program
Ion Source: HESI
Scan method: positive ion mode (ESI+)
Sheath gas pressure: 30 arb
Auxiliary gas pressure: 8 arb
Ion exchange tube: 300 ℃
Auxiliary air temperature: 350 ℃
Table 2. Targes, and Characteristic Ions (*Quantitative Ions)
Results
Table 1. Spiked Aflatoxin B1 In Peanut Recovery
Ordering Information
