biocommaIntroduction
Comparing to traditional SPE cartridges, Copure® HLB LimCartridges for Multi-Residue Analysis of Veterinary Drugremove various interferers such as fats, phospholipids,and pigments more rapidly to reduce the matrix effect. Thepreparation procedures have been simplified to save cost andtime by skipping activation and equilibration.
Biocomma established a UPLC-MS/MS method for detectionof dexamethasone, betamethasone and atropine in Beef withgood recovery and precision for your reference. The recoverieswere 85-110% with RSD less than 10% at low, medium, andhigh spiked concentration levels
Experiment
Extraction
Accurately weigh 2 g of ground Beef into a clean centrifugetube. Add 10 mL of 0.2% formic acid in acetonitrile:water(80:20, v/v) and vortex to mix well. Then, extract by ultrasonictreatment for 15 min, and centrifuged at 8000 r/min for 5 min.The sample is ready for purification.
Purification (Copure® HLB Lim, 200 mg/ 3 mL)
Pipette 1.5 ml supernatant of extracted sample to a Copure®HLB Lim Cartridge. Collect the filtrate and evaporate todryness. Redissolve to 1 mL by 0.1% formic acid in watermethanol (9:1). After filter by nylon membrane, the sample isready for analysis.
Preparation of Standard Curve Solution
Prepare blank sample in the same procedures. After collectingthe eluent, add internal standard solution. Evaporate and dilutethe concentration of 2 μg/L, 10 μg/L, 20 μg/L, 50 μg/L, 100 μg/L and 200 μg/L.
Instrument Conditions
1.Chromatographic Conditions
Instrument: UPLC-MS/MS (Thermo Fisher TSQ Endura)
Chromatographic column: Commasil® Specialized Columns forVeterinary Residues (2.1 mm×100 mm, 3 μm)
Mobile phase A: water (0.1 % formic acid)
Mobile phase B: methanol: acetonitrile = 2:8 (0.1 % formic acid)Flow rate: 0.3 mL/min
Column temperature: 35 ℃
Injection volume: 10 μL
Table 1. Gradient Elution Program
2.Mass Spectrometry Conditions
Ion source: HESI
Electrospray voltage: 3500 V
Sheath gas pressure:40 arb
Auxiliary gas pressure: 10 arb
Ion exchange tube: 380 ℃
Auxiliary air temperature: 350 ℃
Table 2. Targets, Retention Times and Characteristic Ions(*Quantifier Ion)
Results
Table 3. Spiked Recovery Results
Ordering Information
