biocommaExperiment
Extraction and purification of dark-colored fruits and vegetables
Weigh 10.00 g of the sample into a 50 ml centrifugetube. Add 10 ml of acetonitrile and a ceramichomogenizer, and shake vigorously for 1 min. Add 4 g ofMgSO4, 1 g of NaCl, 1 g of trisodium citrate, and 0.5 g ofdisodium citrate(Cat.No.COQ050010CH). After vigorousshaking for 1 min, centrifuge at 5000 r/min for 5 min.Quantitatively transfer 6 mL of the supernatant to acentrifuge tube containing drying agents and purificationmaterials ( 900 mg anhydrous MgSO4, 150 mg PSA, and15 mg GCB)(Cat.No.COQ015020H) . Vortex for 1 min.Centrifuge at 7500 r/min for 2 min. Filter the supernatantthrough a microporous membrane for further analysis.
Extraction and purification of tea
Weigh 2.00 g of tea into a 50 mL centrifuge tube. Add10 mL of water. Vortex to mix well, and let it stand for 30min. Add 15 mL of acetonitrile-acetic acid solution anda ceramic homogenizer, and shake vigorously for 1 min.Add 6 g of MgSO4 and 1.5 g of sodium acetate. (Cat.No.COQ050020CH)After vigorous shaking for 1 min,centrifuge at 7500 r/min for 5 min. Quantitatively transfer8 mL of the supernatant to a centrifuge tube containingdrying agents and purification materials ( 1200 mg ofMgSO4, 400 mg of C18, 400 mg of PSA, and 200 mgof GCB) (Cat.No.COQ015047H). Vortex for 1 min.Centrifuge at 7500 r/min for 2 min. Filter the supernatantthrough a microporous membrane for further analysis.Filter the supernatant through a microporous membranefor further analysis.
Extraction of Standard Curve Solution
Prepare blank sample in the same procedures. Add mixedstandard to dilute to the concentration of 0.002 mg/L,0.005 mg/L, 0.01 mg/L, 0.02 mg/L, 0.05 mg/L and 0.1 mg/L. Based on the instrument’s performance and testingrequirements, select at least 5 concentrations for LC-MSdetermination. Use the peak area of the mass chromatogramfor pesticide quantification as the vertical axis and thecorresponding matrix-matched standard working solution’smass concentration as the horizontal axis to plot the matrixmatched standard working curve.
1.Chromatographic conditions
Equipment: Tandem Quadrupole Linear Ion Trap MassSpectrometer AB SCIEXQTRAP4500
Chromatographic column: ACQUITY UPLC BEH C18Column 2.150mm, 1.7m
Column temperature: 40 ℃
Injection volume: 1 μL
Table 1. Gradient Elution Program
2.Mass Spectrometry Conditions
Detection mode: MRM
Table 2. Ion Source Control Conditions
Table 3. Targes Characteristic Ions (*Quantifier Ion)
